Enhanced gene transfection efficiency by low-dose 25 kDa polyethylenimine by the assistance of 1.8 kDa polyethylenimine

Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine

We mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical methods. Their in vivo transfection efficiency, specificity, and biodistribution were investigated. N-Ethylation improve...

Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine Citation

The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. We mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical m...

Enhanced gene transfection capability of polyethylenimine by incorporating boronic acid groups.

Phenylboronic acid-modified PEI was prepared by the reaction of 1800 Da PEI with 4-(bromomethyl)phenylboronic acid. It is much more effective than unmodified PEI for gene delivery. The covalently incorporated boronic acid groups achieve the greatly enhanced gene delivery efficiency partially through improving condensation ability to DNA, and partially through facilitating cell uptake due to int...

The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine

Transfection of insect cell lines using polyethylenimine.

Insect cell lines have been widely used in recombinant baculovirus expression systems and transient gene expression studies. Critical to these applications have been the transfection of foreign DNA. This has been frequently done using labor intensive and cytotoxic liposome-based transfection reagents. In the current study we have optimized a new kind of polyethylenimine-based DNA transfection r...